The 2nd International Workshop on Artificial Intelligence for Neuroscience (IWAIN 2025) aims to bring together researchers and practitioners from the fields of artificial intelligence and neuroscience to discuss the latest advancements, challenges, and opportunities at the intersection of these two rapidly evolving disciplines. This workshop provides a platform for interdisciplinary collaboration, fostering innovative approaches that leverage AI to enhance our understanding of the brain and to develop novel neuro-technologies that aid in tasks such as monitoring, diagnosis, data generation, and more.